Mouse R spondin 1/RSPO1 Protein 3547

Additional Information:
inaccessionQ9Z132|express systemHEK293|product tagC-His|purity> 95% as determined by Tris-Bis PAGE|backgroundThe R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated ‘thrombin-sensitive protein.|molecular weightThe protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.|available size100 g, 500 g|endotoxinLess than 1EU per g by the LAL method.|Mouse R spondin 1/RSPO1 Protein 3547proteinSize and concentration100, 500g and lyophilizedFormLyophilizedStorage InstructionsValid for 12 months from date of receipt when stored at -80C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.Storage bufferShipped at ambient temperature.Purity> 95% as determined by Tris-Bis PAGEtarget relevanceThe R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated ‘thrombin-sensitive protein.Protein namesR-spondin-1 (Cysteine-rich and single thrombospondin domain-containing protein 3) (Cristin-3) (mCristin-3) (Roof plate-specific spondin-1)Gene namesRspo1,Rspo1Protein familyR-spondin familyMass10090DaFunctionActivator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes (PubMed:21693646). Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination (By similarity). Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (By similarity).Subellular locationSecreted. Nucleus. Note=Seems to mainly localize to nucleoli.TissuesExpressed in the dorsal part of the neural tube on 10 and 12 dpc, especially in the boundary region between roof plate and neuroepithelium. This expression is enhanced in the rostral part. Also expressed in other tissues such as truncal region neighboring forelimbs and mesenchymal tissues around the nasal cavity.StructureInteracts with ZNRF3; promoting indirect interaction between ZNRF3 and LGR4 and membrane clearance of ZNRF3. Identified in a complex composed of RNF43, LGR5 and RSPO1 (By similarity). Interacts with the extracellular domain of FZD8 and LRP6. It however does not form a ternary complex with FZD8 and LRP6. Interacts with WNT1. Binds heparin. Interacts with LGR4, LGR5 and LGR6 (PubMed:16543246, PubMed:21693646). Interacts (via FU repeats) with KREM1 (By similarity).Post-translational modificationC-, and N-glycosylated. N-glycosylation at Asn-137, negatively influences its secretion and enhancing effect on Wnt/beta-catenin signaling. C-mannosylation at Trp-156 by DPY19L3 is required for its secretion an regulates the enhancing activity of Wnt signaling.DomainThTarget Relevance information above includes information from UniProt accession: Q9Z132The UniProt Consortium|