Name :
ABHD14B Protein
Description :
ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.
Species :
Human
Uniprotkb :
E. coli
Tag :
His
Synonyms :
CIB, abhydrolase domain containing 14B, HEL-S-299
Construction :
A DNA sequence encoding the mature form of human ABHD14B (Q96IU4-1) (Met1-Gln210) was expressed with a polyhistide tag at the N-terminus.
Protein Purity :
> 96 % as determined by SDS-PAGE
Molecular Weight :
Approxiamtely 24.2 kDa
Endotoxin :
Please contact us for more information.
Formulatione :
Supplied as sterile PBS, 20% glycerol, 50mM Arg, 0. 1% Tween20, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping :
Solution. It is shipped out with blue ice.
Research Background :
ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.
References and Literature :
1. Mehrle A,et al.(2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8. 2. Wan D,et al.(2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9. 3. Wiemann S,et al.(2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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