HK1 Enzyme

Description:
|Introduction Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic |E. coli-derived. A single, non-glycosylated, polypeptide chain fused to His tag at the N-terminal encoding the sequence of 937 amino acids and having a molecular mass of 104.6 kDa. Specific activity is 7-8 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C. Purity > 90%, by RP-HPLC and SDS-|The protein (1 ug/ml) contains 20 mM Tris pH8.0 and 10% glycerol. Shipping: The product is shipped at a|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. HK1 Antibodies: HK1 Monoclonal Antibody