Hydrogels with antioxidative and antibacterial properties have emerged as promising candidates for wound dressings to accelerate tissue regeneration. In this study, a series of reduced polydopamine nanoparticles (rPDA NPs) incorporated oxidized dextran/chitosan hybrid hydrogels were developed to leverage the excellent antioxidant activity and inherent antibacterial function of rPDA NPs. The physicochemical characteristics of the hydrogels were systematically evaluated using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and rheological measurements. Results confirmed that rPDA NPs exhibited superior free radical scavenging capacity compared to untreated PDA NPs, which significantly enhanced the antioxidant performance of the hydrogel matrix. The hybrid hydrogels demonstrated rapid gelation via Schiff base crosslinking between oxidized dextran and chitosan, forming a biocompatible and mechanically stable network. Additionally, the incorporation of rPDA NPs improved the swelling ratio and water vapor transmission rate (WVTR), ensuring an optimal moist yet breathable microenvironment conducive to wound healing. In vitro assays revealed that the hydrogels effectively protected human umbilical vein endothelial cells (HUVECs) against hydrogen peroxide-induced oxidative stress, as evidenced by reduced intracellular ROS levels and malondialdehyde (MDA) content. Furthermore, live/dead staining and Alamar Blue assays confirmed high cytocompatibility across all formulations. The antibacterial efficacy was validated through contact inhibition tests and zone of inhibition (ZOI) assays, showing significant suppression of Staphylococcus aureus growth.17406-45-0 MedChemExpress Scanning electron microscopy (SEM) imaging revealed morphological disruption of bacterial cells upon interaction with the hydrogels.67-68-5 manufacturer In vivo evaluation in an infected full-thickness wound model on SD mice demonstrated that the rPDA-containing hydrogel (GelNP-05) accelerated wound closure, reduced inflammation, promoted collagen deposition, and enhanced neovascularization compared to control groups.PMID:29939565 Histological analysis including H&E, Masson’s trichrome, and VEGF immunostaining supported these findings, indicating robust tissue regeneration. These results collectively demonstrate that rPDA NPs-incorporated oxidized dextran/chitosan hydrogels possess integrated antioxidant, antibacterial, and biocompatible properties, making them highly effective for managing infected wounds and promoting rapid healing. This strategy offers a versatile platform for designing next-generation smart wound dressings without requiring additional antioxidants or toxic agents.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
