Rabbit anti-BCL-XL Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. |Synonym Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCL2L |Host Rabbit |Specificity BCL-XL Antibody detects endogenous levels of total BCL-XL. |Species Reactivity Human, Mouse, Rat |Predicted Reactivity Pig, Sheep, Dog, Bovine |Applications WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 |Immunogen A synthesized peptide derived from Human BCL2L1(Accession Q07817), corresponding to amino acid residues T47-S73. |Properties |Form Liquid |Concentration 1mg/ml |Purification The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |Clonality Monoclonal Antibody |Clone S-487-28 |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |Target |Background B-cell lymphoma-extra large (Bcl-xL), encoded by the BCL2-like 1 gene, is a transmembrane molecule in the mitochondria. It is a member of the Bcl-2 family of proteins, and acts as an anti-apoptotic protein by preventing the release of mitochondrial contents such as cytochrome c, which leads to caspase activation and ultimately, programmed cell death. Bcl-xL is a major survival factor responsible for an estimated half of the total survival “signal” proerythroblasts must receive in order to survive and become red cells. Bcl-xL promoter contains GATA-1 and Stat5 sites. This protein accumulates throughout the differentiation, ensuring the survival of erythroid progenitors. Similar to other Bcl-2 family members, Bcl-xL has been implicated in the survival of cancer cells by inhibiting the function of p53. |tissuespecificity Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. |Post-translational modifications Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. |Cellular localization Cytoskeleton;Cytosol;Endoplasmic reticulum;Mitochondrion;Nucleus; |UniProt Q07817 | Western blot analysis of BCL-XL expression in UV treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.