Istriau, 7000 Mons, Belgium; [email protected] (C.H.); Estelle.
Istriau, 7000 Mons, Belgium; [email protected] (C.H.); [email protected] (E.L.); [email protected] (R.N.M.); [email protected] (L.V.E.) Laboratoire de G omique, Bioinformatique et Chimie Mol ulaire (EA 7528), Equipe Chimie Mol ulaire, Conservatoire National des Arts et M iers (Cnam), HESAM Universit 2 Rue Cont 75003 Paris, France; [email protected] (G.H.); [email protected] (C.G.); [email protected] (M.P.) Center for Microscopy and Molecular Imaging (CMMI), 8 Rue Adrienne Boland, 6041 Gosselies, Belgium Correspondence: [email protected]; Tel.: 32-65-Abstract: NMR is often a potent characterization tool and we propose to study the surface of -Irofulven Protocol Silica or bismuth nanoparticles devoted to medical applications so as to evidence the covalent grafting of organic molecules on their surface. For that aim, DOSY experiments are particularly useful and let for the discrimination of molecules interacting strongly together with the nanoparticle surface from molecules simply weakly adsorbed at the surface. We had been able to characterize completely the surface of diverse silica and bismuth nanoparticles.Citation: Henoumont, C.; Hallot, G.; Lipani, E.; Gomez, C.; Muller, R.N.; Vander Elst, L.; Port, M.; Laurent, S. Characterization of Organic Molecules Grafted to Silica or Bismuth Nanoparticles by NMR. Appl. Nano 2021, two, 33043. https:// doi.org/10.3390/applnano2040024 Academic Editor: Angelo Maria Taglietti Received: 29 June 2021 Accepted: 15 October 2021 Published: four NovemberKeywords: nanoparticles surface; DOSY; NMR1. Introduction Nanoparticles (NP) are more and more explored for applications in the healthcare field. They’re able to be applied as drug delivery systems but also as contrast agents for unique imaging strategies. We are able to cite silica-based nanoparticles, liposomes, polymersomes or inorganic NP like iron oxide NP, gold NP, silver based NP, zinc oxide NP, bismuth NP, quantum dots, and so forth. [1]. A vital situation for the use of NP in the medical field would be to have the ability to derivatize the surface in order to receive stable suspensions in aqueous media and buffer as well as to vectorize the NP for targeting, meaning that we’ve got to possess robust tools to characterize their surface, and more precisely, to Nitrocefin Biological Activity attest from the covalent grafting from the ligands of interest to their surface. Nuclear Magnetic Resonance (NMR) is a strong tool to figure out if an organic ligand is covalently attached to a nanoparticle or if it can be, on the contrary, merely adsorbed at the surface on the nanoparticle, with an exchange taking location in between the free of charge along with the bound type of the ligand. Indeed, in addition for the classical 1D and 2D NMR spectra, diffusion ordered spectroscopy (DOSY) experiments are especially valuable for these type of investigations [2]. It enables the determination of your diffusion coefficients from the solution species and will hence permit to discriminate in between covalently grafted ligands and adsorbed ligands. Indeed, when the ligand is covalently attached towards the nanoparticle, its diffusion coefficient are going to be equal to that in the nanoparticle. Around the contrary, in the event the ligand is basically adsorbed on the nanoparticle surface, an average diffusion coefficient among these of your free and from the bound ligand should be observed due to the fast exchange in between these two species (Equation (1)). Dobs = PB DB (1 – PB )DF (1)Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps.
