Nces and Peking Union Medical College, , USA; cChinese Academy of Health-related Sciences and Peking Union Healthcare College, chengdu, China (People’s Republic)Introduction: Evidences recommended that exosomes can transfer genetic material between cells, like viral nucleic acids, proteins or miRNAs which can mediate transmission of viruses for instance HBV or HCV. It truly is known that platelet-derived exosomes constitute the big fraction in the circulating plasma which can participate in haemostasis, immunity and improvement. Regardless of whether the virus infected platelet-derived exosomes also can promote the transmission of virus has not been reported. The hepatitis E virus (HEV) is among the most common causes of acute hepatitis worldwide. Current research have shown that the exosomes secreted by HEV-infected cells had been infectious. Our research have confirmed that HEV can infect platelets, as a result we performed this study to prove if exosomes secreted by CD281/TLR1 Proteins Biological Activity platelets infected with HEV are also infectious, thereby additional promoting the transmission of HEV. Strategies: An in vitro model of HEV-infected platelets were established by HEV-G3 virus strain and washed human platelets as well as the exosomes had been isolated from HEV-infected and uninfected platelet by differential centrifugation and magnetic bead separation. Exosomes have been characterized by Western Blot and TEM, and quantitated by NTA. qRT-PCR and ELISA were utilized to detect HEV RNA and proteins in exosomes. Good exosomes have been utilised to infect PLC/PRF/5 cells, observing the changes of HEV RNA and proteins within one particular month. Results: The in vitro model of HEV-infected platelets was effectively established. The concentration of exosomes secreted by HEV-infected platelets was greater than uninfected platelets. Exosomes isolated from HEV-infected platelets contained HEV RNA andJOURNAL OF EXTRACELLULAR VESICLESproteins. HEV RNA and proteins were detected in cells and supernatant of PLC/PRF/5 cells infected with constructive exosomes, and the concentration of which increased after the culture of one month. Summary/Conclusion: Our study showed that HEV can market the secretion of platelet exosomes and these vesicles can establish a productive infection which recommended that the exosomes secreted by platelets not simply play a function in haemostasis, immunity and improvement, but additionally play a non-negligible part in the transmission of the virus. Funding: 1.CAMS Innovation Fund for Health-related Sciences (CIFMS2016-I2M-1-018) 2. Supported by the Basic Analysis Funds for the Central Universities(Item No:3332018125)roles in HBV hepatitis through the multiorgan association of liver, bone marrow and gut. Funding: AMED hepatitis grant.PF05.HIV-1 Nef mediated Hck kinase activation triggers PTPRF Proteins MedChemExpress loading of TACE into EVs within a ceramide-dependent manner Zhe Zhao, Riku Fagerlund and Kalle Saksela University of Helsinki, Helsinki, FinlandPF05.Multi organ association mediated by extracellular vesicles secreted from HBV constructive hepatocyte Ai Kotania and Masatoshi KakizakibaTokai University, Isehara, Japan; bTokai University, School of Medicine, Division of Gastroenterology, Iisehara, JapanIntroduction: Hepatitis B virus (HBV) infected hepatocytes secreted extracellular vesicles which include virion, exosome and incomplete virions like hallow particles which have only HBs viral antigens but neither capsid and HBV genome. We discovered that the EVs are taken by monocyte/macrophage which upregulates PDL1, immune checkpoint molecule. (Kakizaki et al PLOS one particular in press).