Otillin-1 and Alix. As outlined by the NTA the EVs were heterogeneous in size. Summary/Conclusion: HOK-16B cells released EVs that have basic EV markers. The EVs derived from HOK-16B infected with periodontopathogen really need to analyse and confirm the biological function to other cells. CD115/M-CSF R Proteins Recombinant Proteins Funding: This function was supported by National Study Foundation of Korea grants (No. NRF-2018R1A5A2 024418 and NRF-2018R1A2A2A05018558).PF01.Air pollution effects around the clinical course of autoimmune diseases: the function of extracellular vesicles Mirjam Hoxhaa, Tommaso Schioppob, Simona Iodicea, Laura Pergolia, Nicola Ughib, Luca Ferraria, Francesca Ingegnolib and Valentina BollatiaaUniversity of Milan, Division of Clinical Sciences and Community Overall health, Milan, Italy; bDivision of Clinical Rheumatology, G. Pini Hospital, Milano, ItalyPF01.Isolation of EVs derived from human oral keratinocytes Younggap Lim and Bong-Kyu Choi Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University, Jongno-gu, Seoul, Republic of Korea, Seoul, Republic of KoreaIntroduction: Oral keratinocytes are the 1st defense line against external environments which include chemical agents, microbes and physical components. Stimulated oral keratinocytes produce cytokines/chemokines to modulate regional inflammatory status. According to current researches, not only cytokines/chemokines but extracellular vesicles (EVs) also regulate immune response. Consequently, we hypothesized that oral keratinocytes release EVs and those EVs could modulate immune response within the gingival tissue. Procedures: EVs were isolated from human oral keratinocytes (HOK-16B) by ultracentrifugation (UC) and commercial EVs isolation kit and analysed by western blotting and Nanoparticle Tracking Analysis (NTA). Outcomes: To exclude EVs originated from cell culture medium, we compared 3 unique keratinocyte culture media, then we chose medium that contained theIntroduction: Autoimmune diseases (Ads) are characterized by the body’s intolerance to self-antigens. The reason for autoimmunity is still unknown. Nonetheless, it truly is typically accepted that Ads could possibly be triggered by environmental things capable to increase inflammation. In current years, extracellular vescicles (EVs) have already been described to play a vital role each in Advertisements pathogenesis and environmental toxicants, such as particulate matter (PM). The aim of our study should be to evaluate PM effects on EV release in Advertisements. Procedures: We recruited 24 patients with Advertisements (12 Rheumathoid Arthritis, RA and 12 Systemic B7-H4 Proteins supplier Sclerosis, SSc) and 12 individuals with Osteoarthritis (OA), a nonautoimmune inflammatory disease taken as control. Plasma EVs have been analysed by Nanosight and flow cytometry following labelling with the following markers: CD14+ (monocyte), CD61+ (platelet), CD25+ (T-reg), ERVWE1+ (human endogenous retrovirus W), HLAG + (human leukocyte antigen G). PM10 and PM2.five concentrations at the residency of every subject were obtained in the regional air excellent monitoring network. Outcomes: The boost of PM2.five led to a decrease of MVs CD14+ ( = -0.13; p 0.01) and CD61+ ( = -0.08; p = 0.05) in RA, of ERVWE1+ in each SSc ( = -0.10; p = 0.01) and OA ( = -0.09; p = 0.01), and of HLA+ ( = -0.12; p 0.01) only in SSc. Equivalent results were observed analyzing PM10 exposure. Analysis of EVs concentration as outlined by theirISEV2019 ABSTRACT BOOKdimensions showed a adverse association in the size range of exosomes (632 nm) in RA and SSc in comparison with OA (p 0.05). Finally, we obse.