Of stimulation (Fig. S3), except for TNF-a, where also monocytes are
Of stimulation (Fig. S3), except for TNF-a, where also monocytes are likely to contribute for the TNF-a detected.—————————————————————————— “Figure 3. RhuDex1 impairs cytokine release of WO-LP and PB T cells. Cytokine concentrations have been measured in culture supernatants collected immediately after 24 h of stimulation of WO-LPL, or LPS-activated PBMO co-cultured with non-adherent PBL. RhuDex1 and Abatacept were added in the beginning of culture. The mean cytokine responses of each donor within the presence of inhibitors were normalized to the responses with out inhibitors (medium, set to one hundred ). For (A) IL-17, (B) IFN-g, (C) IL-2, and (D) TNF-a, the upper graph of every panel depicts responses in WO-LPL (five donors, numbered 1). The reduce graph indicates responses in PBL of four allogeneic (allo, numbered I V) and three donors autologous (auto) to WO-LPL. Information points for every single donor are shown in individual colors, as well as the imply SD of all information points in every single condition is shown as columns and error bars. P 0.05; P 0.01; P 0.001; P 0.0001. Med, medium control, Aba, Abatacept, Rhu, RhuDex1, inhibitor concentrations in mgmL.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.CD80 Blockage by RhuDex1 HIV manufacturer Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Acytokine secretion [ ]250 200 150 one hundred 50 0 300IL-17 WO-LPL1 2 three 4IL-17 PBLI allo II allo III allo IV allo two auto 3 autocytokine secretion [ ]200 150 one hundred 50 0 Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.five CD3 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 CD4 auto 5 autoBcytokine secretion [ ]IFN- WO-LPL0IFN- PBLcytokine secretion [ ]0 Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.5 CDFigure 3. Continued.Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.five CD2014 The Authors. Immunity, Inflammation and Disease Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationCcytokine secretion [ ]250 200 150IL-2 WO-LPL1 two 3 450 0IL-2 PBLI allo II allo III allocytokine secretion [ ]IV allo 2 auto three auto4 auto 5 auto0 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.5 Med Aba ten Aba 1 Rhu 20 Rhu three Rhu 0.five CD2 CD3Dcytokine secretion [ ]TNF- WO-LPL0 250 200 150 100 50 0 Med Aba ten Aba 1 Rhu 20 Rhu 3 Rhu 0.5 CDFigure three. Continued.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.TNF- PBLcytokine secretion [ ]Med Aba 10 Aba 1 Rhu 20 Rhu three Rhu 0.5 CDCD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationA.-K. Heninger et al.Figure four. Intracellular cytokine expression of CD4or CD8WO-LP and PB T cells after activation. (A) Proportion ( ) of CD4and CD8T cells among CD3T cells in PBL (three allogeneic donors) and WO-LPL (2 tissue donors). (B) Representative dot-plots (donor III) displaying the intracellular cytokine expression (IL-17, IL-2, IFN-g and TNF-a) of CD4WO-LP T cells (left panel) and CD8WO-LP T cells (appropriate panel), or (C) of CD4PB T cells (left panel) and CD8PB T cells (ideal panel), as detected within the absence of stimulation, or six h of anti-CD3, or anti-CD2 stimulation.2014 The Authors. Immunity, Inflammation and Illness Published by John Wiley Sons Ltd.A.-K. Heninger et al.CD80 Blockage by RhuDex1 Reduces Intestinal T Cell ActivationAcytokine expression [ ]CD4 WO-LP T CDK14 Purity & Documentation cells150 one hundred 50 0 150 one hundred 50 0 150 one hundred 50 0 150 100 50 0 TNFIFNIL-2 IL-Bcytokine expression [ ]CD4 PB T cells150 100 50 0 150 one hundred 50 0 150 one hundred 50 0 150 one hundred 50 0 TNFIFNIL-2 IL-cytokine expression [ ]cytokine expression [ ]cytok.
